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“ 即用型 iPSC 肝类器官 ”是以人诱导多能干细胞（iPSC, induced pluripotent stem cells）为起点细胞，基于肝脏发育进程的关键分子信号，凭借 iPSC 谱系共分化技术诱导分化形成。iLiver 具有更接近人真实肝脏的细胞谱系及结构特征，为肝脏发育机制的探索、肝病模拟、药物开发与评价等研究提供可靠的模型工具。
| 遗传信息 | |||
| • 类器官类型： | • 来源： | • 性别： | |
| 肝类器官 | 成纤维细胞 | 女 | |
| • 捐助者状态： | • 年龄： | ||
| 健康 | 20 |
Characteristics
Day-25 iLivers represent uniformly sized 3D spheroids, which consists of hepatocytes (ALB+), cholangiocytes (CK7+), Kupffer cells (CD68+) and hepatic stellate cells (VIM+), as identified by immunofluorescence. Scale bar = 100 μm.
Application of iLiver Organoids
Modeling NASH using day-24 iLivers manifested excessive accumulation of fibrosis (a-SMA staining), along with an increased secretion level of clinical fibrosis marker PIIINP by ELISA analysis.
Drug Metabolism in iLivers
Tadokoro T, Murata S, Kato M, et al. Human iPSC–liver organoid transplantation reduces fibrosis through immunomodulation[J]. Science Translational Medicine, 2024, 16(757): eadg0338.
Zhang C J, Meyer S R, O’Meara M J, et al. A human liver organoid screening platform for DILI risk prediction[J]. Journal of hepatology, 2023, 78(5): 998-1006.
Guan Y, Enejder A, Wang M, et al. A human multi-lineage hepatic organoid model for liver fibrosis[J]. Nature communications, 2021, 12(1): 6138.
Wu D, Chen X, Sheng Q, et al. Production of functional hepatobiliary organoids from human pluripotent stem cells[J]. International journal of stem cells, 2021, 14(1): 119-126.
Wu F, Wu D, Ren Y, et al. Generation of hepatobiliary organoids from human induced pluripotent stem cells[J]. Journal of Hepatology, 2019, 70(6): 1145-1158.
